(1) Colloidal Gold’s structure
Colloidal Gold, also known by the name gold sol, is the suspension of particles made from gold after the original salt is removed. The essential gold core, also known as the atomic gold Au, is found within colloidal particles of colloidal. It’s surrounded by a double layer of ions. For the maintenance of the suspension state free of colloidal gold between sols, the layer with negative ions (AuC122-) is connected to it. Colloid gold particles’ primary gold core isn’t ideal for spherical symmetry. Although the colloidal gold particles smaller than 30nm are usually spherical, those larger are almost all elliptical. A electron microscope allows you to observe colloidal-gold’s particle morphology.
(2) Some characteristics of colloidal Gold
1. Colloidal nature. Most colloidal silver particles measure between 100 and 1000 nm in diameter. These tiny gold particles can be suspended in the liquid in an even distribution to form a colloidal solution. Colloid gold therefore has many of the same properties as colloids. In order to break the solid state, the electrolyte could destroy the colloidal hydration layer. Certain macromolecular molecules, including proteins, can protect and improve the stability of colloidal golden.
It is an often used labeling technology. It’s a brand new immunolabeling technique that utilizes colloidal gold for tracer markers of antigens. It offers many advantages. It’s been extensively used in biological studies for many years. The label is applicable to almost every immunoblotting technique used clinically. This label is also useful in electron microscopy (fluid cytometry), immunology, molecular biology, biochips, and other applications.
Chloroauric acid (HAuCl4) is used to create colloidal gold. When reducing agents are applied, such as whitephosphorus or ascorbic acids, sodium citrate, tannic Acid, etc., the resulting particles can be produced into tiny gold particles. It then becomes a stable state of static electricity. The stable state of colloidal colloidal is formed by a negatively-charged hydrophobic glue. It can form strong bonds when colloidal golden is positively charged. Since this bond is electrostatic it doesn’t affect the biological attributes of the protein.
A coating procedure that allows proteins to be adsorbed onto colloidal-gold particles is called Colloidal Gold Labeling. Adsorption can be explained by the existence of a negative charge on the surface colloidal-gold particles. The negatively charged group of the protein forms strong bonds with them due to electrostatic attraction. Chloroauric Acid can be used to make colloidal silver particles, which are of various sizes. This particle is spherical and has a strong adsorption ability for proteins. It is non-covalently compatible with staphylococcal Protein A, immunoglobulins. Because of this, it is a great tool in both basic research and clinical experimentation.
Common detecting technology
Immunocolloid Gold Staining
Staining cell sections and suspensions of tissue can be done with colloidal -labeled antibodies. Also, the silver developer is made from colloidal -labeled silver particles.
Immunocolloid gold electron microscope staining
The antibodies and anti-antibodies can be combined with negatively stained virus samples, or extremely thin sections of tissue. It can be used in the detection and observation of viruses morphology.
Filtration with Dot immunogold
A microporous membrane is used as a carrier. First, spot any antigen or antibody. Next, add the sample for testing. Finally, wash the surface with colloidal-labeled antibody.
Colloidal gold immunochromatography
The membrane is coated with the antibody specific to it in a rectangular shape. On the binding surface, the colloidal-gold labeling reagent or monoclonal antibody is adsorbed. To test the specimen, add it to the bottom of the test strip. With the naked eye, you can observe the test strip’s color development results. The diagnostic test strips made from this method are very simple to use.
The rapid development of standard gold standard technology
Once the antibody has been attached to one particular region of the acid cell membrane, it is then fixed. If one side of the dried acidcellulose is placed in the sample (urine/serum), the sample will travel along the membrane because of capillary movement Move. Once the antibody has been fixed to the membrane, any antigen found in that region will be bound to it. The addition of gold particles gives rise to high electron densities in the vicinity of the gold-labeled proteins. This was visible by the naked eye when red spots appeared as a result of the accumulation of these markers at their respective ligands. This is the basis of rapid label detection. If the sensitiveness and specificity, of antigens, proteins chimeras or monoclonal antibody, polyclonal antibody, antigens, colloidal gold, can be reached the highest standards, then the production of fast and accurate reagents will not be possible. This is gold. It’s the best standard for the quality and safety of standard reagents.
Cataniadagiocare, Cataniadagiocare advanced Material Tech Co., Ltd., is a Colloidalgold producer who has more than twelve years of chemical products research, development and manufacturing experience. Please contact us to inquire about high quality Colloidal golden.